Accurate and simple determination of oxcarbazepine in human plasma and urine samples using switchable-hydrophilicity solvent in GC-MS

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Erarpat S., Bodur S., Ayyıldız M. F., Günkara Ö. T., Erulaş F., Chormey D. S., ...More

BIOMEDICAL CHROMATOGRAPHY, vol.34, no.10, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 10
  • Publication Date: 2020
  • Doi Number: 10.1002/bmc.4915
  • Journal Name: BIOMEDICAL CHROMATOGRAPHY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, EMBASE, MEDLINE, Veterinary Science Database
  • Keywords: gas chromatography-mass spectrometry, human plasma, oxcarbazepine, switchable hydrophilicity solvent, urine, LIQUID-LIQUID MICROEXTRACTION, 6 ANTIEPILEPTIC DRUGS, PHASE MICROEXTRACTION, SIMULTANEOUS QUANTIFICATION, ELECTROSPRAY-IONIZATION, MASS-SPECTROMETRY, HPLC-UV, CARBAMAZEPINE, CHROMATOGRAPHY, METABOLITES
  • Yıldız Technical University Affiliated: Yes

Abstract

This work presents a sensitive and rapid analytical method for the determination of oxcarbazepine in human plasma and urine samples. A vortex-assisted switchable hydrophilicity solvent-based liquid phase microextraction (VA-SHS-LPME) was used to preconcentrate oxcarbazepine from the samples before the determination by gas chromatography mass spectrometry. The switchable hydrophilicity solvent was synthesized by protonatingN,N-dimethylbenzylamine with carbon dioxide to make it totally miscible with an equivalent volume of water. Parameters of the VA-SHS-LPME method including volume of switchable hydrophilicity solvent, concentration/volume of sodium hydroxide and vortex period were systematically optimized. Under the optimum conditions, good linearity ranging from 27.03 to 353.47 mu g/kg was obtained for the analyte. Limit of detection and quantitation values were found to be 6.2 and 21 mu g/kg (mass base), respectively. The relative standard deviation was calculated as 6.9% for six replicate measurements of the lowest concentration of the calibration plot. Satisfactory recovery results were calculated in the range of 97-100% for human plasma and urine samples spiked at five different concentrations.