Accurate and simple determination of oxcarbazepine in human plasma and urine samples using switchable-hydrophilicity solvent in GC-MS


ERARPAT S. , Bodur S., Ayyıldız M. F. , GÜNKARA Ö. T. , Erulaş F., Chormey D. S. , ...Daha Fazla

BIOMEDICAL CHROMATOGRAPHY, cilt.34, 2020 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 34 Konu: 10
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/bmc.4915
  • Dergi Adı: BIOMEDICAL CHROMATOGRAPHY

Özet

This work presents a sensitive and rapid analytical method for the determination of oxcarbazepine in human plasma and urine samples. A vortex-assisted switchable hydrophilicity solvent-based liquid phase microextraction (VA-SHS-LPME) was used to preconcentrate oxcarbazepine from the samples before the determination by gas chromatography mass spectrometry. The switchable hydrophilicity solvent was synthesized by protonatingN,N-dimethylbenzylamine with carbon dioxide to make it totally miscible with an equivalent volume of water. Parameters of the VA-SHS-LPME method including volume of switchable hydrophilicity solvent, concentration/volume of sodium hydroxide and vortex period were systematically optimized. Under the optimum conditions, good linearity ranging from 27.03 to 353.47 mu g/kg was obtained for the analyte. Limit of detection and quantitation values were found to be 6.2 and 21 mu g/kg (mass base), respectively. The relative standard deviation was calculated as 6.9% for six replicate measurements of the lowest concentration of the calibration plot. Satisfactory recovery results were calculated in the range of 97-100% for human plasma and urine samples spiked at five different concentrations.