Solid phase extraction of beta-sitosterol and alpha-tocopherol from sunflower oil deodorizer distillate using desilicated zeolite


Karaoglu O., Alpdogan G. , Ozdemir I. S. , Ertas E.

GRASAS Y ACEITES, vol.71, no.3, 2020 (Journal Indexed in SCI) identifier

  • Publication Type: Article / Article
  • Volume: 71 Issue: 3
  • Publication Date: 2020
  • Doi Number: 10.3989/gya.0570191
  • Title of Journal : GRASAS Y ACEITES

Abstract

In this study, the efficiency of using zeolite-based adsorbents in a solid phase extraction (SPE) procedure of alpha-tocopherol and beta-sitosterol isolation from Sunflower Oil Deodorizer Distillate (SuDOD) without pre-treatment was investigated. The results showed that 99.2% a-tocopherol and 97.3% beta-sitosterol were successfully isolated as pure fractions from SuDOD, when desilicated ZSM-5-type zeolite (DSiZSM-5) was used as adsorbent on a SPE. A simple and rapid HPLC method for simultaneous alpha-tocopherol and beta-sitosterol analysis was developed and validated according to AOAC guidelines. It was found that the inclusion of a DSiZSM-5 SPE step increased the precision of the alpha-tocopherol and beta-sitosterol analysis. In conclusion, DSiZSM-5 zeolite was proven to be an efficient adsorbent which can be used not only for the recovery of alpha-tocopherol and beta-sitosterol from SuDOD in industrial scale, but also in a laboratory scale clean-up method prior to the analysis of alpha-tocopherol and beta-sitosterol.