Akademik Veri Yönetim Sistemi
15th INTERNATIONAL MARMARA SCIENCE AND SOCIAL SCIENCES CONGRESS, Kocaeli, Türkiye, 28 - 29 Kasım 2025, ss.33, (Özet Bildiri)
As the eighth most common cancer in women, ovarian cancer is marked by high morbidity and mortality. It is considered the most lethal gynecologic cancer based on five-year survival. The key constraint in ovarian cancer therapy is acquired chemotherapy resistance, necessitating novel therapeutic agents to overcome multidrug resistance. Folic acid is a biomolecule that targets the folate receptor. Folate receptor overexpression on tumor cell surfaces confers a considerable advantage in cancer cells employing passive targeting. The natural polymer chitosan is widely adopted in nanocarrier systems for its biocompatibility and bioadhesion. In addition, PLGA nanocarriers are highly beneficial delivery systems, leveraging their biocompatibility and biodegradability for the efficient encapsulation of both hydrophilic and hydrophobic drugs. Biochanin A, chemically known as 4′-methoxy-5, 7-dihydroxy isoflavone, is an O-methylated isoflavonoid exhibiting estrogenic action, present in several plants including chickpeas, soybeans, peanuts, and red clover, and is commonly utilized in traditional Chinese medicine. Biochanin A isoflavone has antiinflammatory, antioxidant, antimicrobial, neuroprotective, and hepatoprotective activities. This study will investigate at the effect of Biochanin A-loaded folic acid-chitosan coated PLGA nanoparticles on sensitive and PTX-resistant ovarian cancer cells. For this purpose, XTT cell viability test was performed to investigate the antiproliferative effect of Biochanin A compound on L929, A2780 sensitive and A2780/PTX resistant ovarian cancer cells and to determine the effective working concentration. Nanocarriers were created utilizing the nanoprecipitation process and coated with a folic acid-chitosan conjugate. UV-Vis Spectroscopy and FT-IR Analysis were used to validate the folic acid-chitosan conjugation. The size, size distribution and zeta potential of the synthesized nanocarriers were analyzed using the Zetasizer device. The encapsulation efficiency and drug loading capacity of the nanocarriers were also calculated using Liquid Chromatography-High Resolution Mass Spectrometry (Q-TOF-LC-MS). The synthesis and characterization of the nanoparticles have been successfully completed, and future studies will continue with in vitro experiments.