INHIBITION OF CARBONIC ANHYDRASE I AND II WITH TOTAL ANTHOCYANINS EXTRACTED FROM SWEET CHERRY CULTIVARS


Demir T., Gunesli Z., Sonmez F., Bilen C. , Yavuz E., Gencer N.

Environmental Engineering and Management Journal, cilt.14, ss.935-941, 2015 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 14 Konu: 4
  • Basım Tarihi: 2015
  • Doi Numarası: 10.30638/eemj.2015.104
  • Dergi Adı: Environmental Engineering and Management Journal
  • Sayfa Sayısı: ss.935-941

Özet

Total anthocyanins were extracted from six sweet cherry cultivars grown in Sakarya, Turkey. In vitro inhibition effects of the extracted total anthocyanins on purified carbonic anhydrase I and II were investigated using CO2 as a substrate. All the extracted total anthocyanins inhibited the activity of the human carbonic anhydrase (hCA) I and II enzymes. Among all the cultivars, 'Karakiraz' was found to be the most active on both hCA I (IC50 = 30.59 mu g/mL) and hCA II activity (IC50 = 44.52 mu g/mL). Additionally, all the cultivars have higher inhibitory activity on hCA I than hCA II.