INHIBITION OF CARBONIC ANHYDRASE I AND II WITH TOTAL ANTHOCYANINS EXTRACTED FROM SWEET CHERRY CULTIVARS


Demir T., Gunesli Z., Sonmez F., Bilen C. , Yavuz E., Gencer N.

Environmental Engineering and Management Journal, vol.14, no.4, pp.935-941, 2015 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 14 Issue: 4
  • Publication Date: 2015
  • Doi Number: 10.30638/eemj.2015.104
  • Title of Journal : Environmental Engineering and Management Journal
  • Page Numbers: pp.935-941

Abstract

Total anthocyanins were extracted from six sweet cherry cultivars grown in Sakarya, Turkey. In vitro inhibition effects of the extracted total anthocyanins on purified carbonic anhydrase I and II were investigated using CO2 as a substrate. All the extracted total anthocyanins inhibited the activity of the human carbonic anhydrase (hCA) I and II enzymes. Among all the cultivars, 'Karakiraz' was found to be the most active on both hCA I (IC50 = 30.59 mu g/mL) and hCA II activity (IC50 = 44.52 mu g/mL). Additionally, all the cultivars have higher inhibitory activity on hCA I than hCA II.