Accurate and Sensitive Reverse Phase High-Performance Liquid Chromatographic Determination of Arbutin in Blueberries and Characterization of Its Stability in Simulated Gastric Fluid and under Ultraviolet Irradiation

Durak B. Y., Sel S., BAKIRDERE S.

ANALYTICAL LETTERS, vol.53, no.9, pp.1504-1511, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 53 Issue: 9
  • Publication Date: 2020
  • Doi Number: 10.1080/00032719.2019.1710523
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chimica, Communication Abstracts, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.1504-1511
  • Yıldız Technical University Affiliated: Yes


In this study, arbutin, an antibacterial skin-whitening agent was identified and quantified in blueberry samples using a developed high-performance liquid chromatographic (HPLC) method. The best instrumental conditions were obtained by performing step-by-step optimization studies. The highest arbutin signal was observed at a wavelength of 220 nm with the ultraviolet detector. Arbutin was eluted through a C18 column by a mobile phase composed of 89:10:1 (v/v/v) water:methanol:0.10 M hydrochloric acid. The analytical figures of merit were calculated from a calibration plot developed across the concentration range from 0.010 to 250 mg/L under the optimum conditions with a coefficient of determination value of 0.9999. The limits of detection and quantification values were 2.0 and 6.6 mu g/L, respectively. Spiked recovery measurements were used to ascertain the applicability of the analytical method in different blueberry samples, and the obtained recovery values ranged between 93 and 100%. The analyte was exposed to simulated gastric fluid and ultraviolet radiation to ascertain its stability under these conditions.