Cytotoxic and mutagenic potential of juglone: a comparison of free and nano-encapsulated form

Creative Commons License

Erişen S., Arasoğlu T., Mansuroğlu B., Kocaçalışkan İ., Derman S.

Arhiv za Higijenu Rada i Toksikologiju, cilt.71, sa.1, ss.69-77, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 71 Sayı: 1
  • Basım Tarihi: 2020
  • Doi Numarası: 10.2478/aiht-2020-71-3344
  • Dergi Adı: Arhiv za Higijenu Rada i Toksikologiju
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, Greenfile, MEDLINE, Pollution Abstracts, SportDiscus, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.69-77
  • Anahtar Kelimeler: alfalfa, L929 fibroblasts, nanoparticle, PLGA, S. typhimurium, ANTIBACTERIAL ACTIVITY, SEED-GERMINATION, WALNUT LEAF, IN-VIVO, GROWTH, PLGA, NANOPARTICLES, NAPHTHOQUINONES, ASSAY, EXTRACTS
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

© 2020 Semiha Erisen et al., published by Sciendo 2020.Despite its evidenced beneficial herbicidal, antibacterial, antiviral, antifungal, and antioxidant effects, the application of juglone (5-hydroxy-1,4,-naphthoquinone) is limited due to its low water solubility and allelopathic and toxic effects. In recent years, research has aimed to overcome these limitations by increasing its solubility and controlling its release through nanoparticular systems. This is the first study to have synthesised and characterised juglone-loaded polymeric nanoparticles and compared them with free juglone for cytotoxicity in mouse (L929 fibroblasts) and alfalfa cells and for mutagenic potential in Salmonella typhimurium TA98/100. Mouse and plant cells treated with free and nano-encapsulated juglone showed a decrease in cell viability in a dose and time-dependent manner, but this effect was significantly lower with the nano-encapsulated form at lower doses. In the TA98 strain with S9, nano-encapsulated juglone did not exhibit mutagenic effects, unlike the free form. Since all results show that juglone encapsulation with polymeric nanoparticles reduced the toxic and mutagenic effects, it has a promising potential to be applied in medicine, food safety, and agriculture.