Nanopore detection of double stranded DNA using a track-etched polycarbonate membrane

KEÇECİ K., SAN N., Kaya D.

TALANTA, cilt.144, ss.268-274, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 144
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1016/j.talanta.2015.06.005
  • Dergi Adı: TALANTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.268-274
  • Anahtar Kelimeler: Resistive-pulse, Track-etched polymer membrane, DNA, Conically shaped nanopore, Biosensing, SINGLE-MOLECULE DETECTION, RESISTIVE-PULSE, ARTIFICIAL NANOPORE, LABEL-FREE, NANOPARTICLES, NANOPIPETTES, FABRICATION, TRANSPORT, SENSORS
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

We investigate the resistive-pulse sensing of 50-bp DNA using track-etched polycarbonate (PC) nanopores and show the translocation dynamics originating from the electrophoretic transport of DNAs. Conically shaped PC nanopore membranes have been prepared with asymmetric chemical etching technique. We show the potential and concentration dependence of DNA translocation through a PC nanopore. We find that the translocation of DNA scales linearly with both potential and concentration. Additionally, the threshold potential is determined to complete the translocation. Finally, by investigating the current-pulse amplitudes of nanopores with different tip sizes, we show that the nanopore size can be successfully used to distinguish the DNA molecules. These results suggest great promise for the sensing of short DNAs and understanding the dynamics of the translocation process using chemically-etched PC nanopores. (C) 2015 Elsevier B.V. All rights reserved.