Investigation of Mesenchymal Stem Cells Differentiation Under Static Magnetic Field Induction


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Ordu E. , Molo K.

8th INTERNATIONAL MOLECULAR BIOLOGY AND BIOTECHNOLOGY CONGRESS, Afyon, Türkiye, 17 - 20 Aralık 2019, ss.78

  • Basıldığı Şehir: Afyon
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayısı: ss.78

Özet

Mesenchymal stem cells (MSCs) are important biological tools for regenerative medicine or cell therapies. Static Magnetic Field (SMF) is one of the biophysiological stimulants to modulate physiological processes in different cell lines. In vitro conditions, SMFs, according to their intensity and duration, change cellular membrane polarization, oxidative product concentrations, gene expression patterns and cell propagation rates. In this study, human bone marrow derived MSCs were grown under 328 mT SMF by using Neodymium Iron Boron (NdFeB) magnets to investigate SMF influance on their proliferation rates, morphologies, osteogenic differantiation capacity. The proliferation rate was analysed by MTT 24 hours and 6 days after SMF induction. Although preliminary results showed that cells with spindle shapes in SMF treated group gained an oriantation according to magnetic field direction, SMF influence did not significantly changed proliferation rates. It was analyzed and compared alkaline phosphatase (ALP) activity in the cells grown for 14 days under both continual SMF and chemically osteogenic induction. It was observed that ALP activity in this group decreased compared to the control groups. Also, Alizarin Red S staining showed that mineralization decreased in the cells grown under the same conditions compared to control cells. This work was supported by Research Fund of the Yildiz Technical University. Project Number: FYL-2019-3520