Determination of the Optimum Annealing Temperature in the Production of CuO Nanoparticles with Antimicrobial Properties

Oruç Ç., Çelik Bozdoğan A., Kurt Gür G., Aşıkoğlu Bozkurt A., Luş H. M.

NANO, vol.17, no.1, pp.22500141-22500149, 2022 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 17 Issue: 1
  • Publication Date: 2022
  • Journal Name: NANO
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Biotechnology Research Abstracts, Communication Abstracts, Compendex, INSPEC, Metadex
  • Page Numbers: pp.22500141-22500149
  • Yıldız Technical University Affiliated: Yes


A copper oxide (CuO) nanoparticle, a transition metal oxide with a wide variety of easy production

methods, can be used as an antimicrobial agent against various types of bacteria. CuO

nanoparticles were produced by the sol–gel method, annealed and structural characteristics and

antimicrobial properties of these particles were investigated. Single-phase monoclinic of CuO

nanoparticle formation was con¯rmed by X-ray powder di®raction (XRD) spectra, FTIR techniques,

di®erential scanning calorimetry with thermal gravimetry were used to characterize. It

was determined that annealing in the temperature range of 150–900C a®ects both structure and

particle size and antimicrobial characteristics. CuO nanoparticle size was found to be between

about 25–70 nm at 150–900C annealing temperature, which does not have this wide temperature

range in the literature. These results were supported by the TEM micrographs of the CuO

nanoparticles observed at 150C and 900C. The antimicrobial activity of the synthesized

nanoparticles was tested with the disc di®usion assay against Staphylococcus aureus, Escherichia

coli, Agrobacterium tumefaciens, and yeast Pichia pastoris.