Defining a stimuli-response relationship in compensatory lung growth following major resection


RAVIKUMAR P., Yilmaz C., DANE D. M., BELLOTTO D. J., ESTRERA A. S., HSIA C. C. W.

JOURNAL OF APPLIED PHYSIOLOGY, cilt.116, sa.7, ss.816-824, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 116 Sayı: 7
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1152/japplphysiol.01291.2013
  • Dergi Adı: JOURNAL OF APPLIED PHYSIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.816-824
  • Anahtar Kelimeler: pneumonectomy, mechanical stress and strain, deformation, mechanotransduction, morphometry, dog, RETINOIC ACID SUPPLEMENTATION, MEMBRANE DIFFUSING-CAPACITY, PULMONARY EPITHELIAL-CELLS, VIVO MECHANICAL STIMULI, CARDIOPULMONARY ADAPTATIONS, COMPUTED-TOMOGRAPHY, RIGHT PNEUMONECTOMY, GENE-EXPRESSION, NITRIC-OXIDE, ADULT DOGS
  • Yıldız Teknik Üniversitesi Adresli: Hayır

Özet

Major lung resection is a robust model that mimics the consequences of loss-of-functioning lung units. We previously observed in adult canines, following 42% and 58% lung resection, a critical threshold of stimuli intensity for the initiation of compensatory lung growth. To define the range and limits of this stimuli-response relationship, we performed morphometric analysis on the remaining lobes of adult dogs, 2-3 years after surgical removal of similar to 70% of lung units in the presence or absence of mediastinal shift. Results were expressed as ratios to that in corresponding control lobes. Lobar expansion and extravascular tissue growth (similar to 3.8- and similar to 2.0-fold of normal, respectively) were heterogeneous; the lobes remaining next to the diaphragm exhibited a greater response. Tissue growth and capillary formation, indexed by double-capillary profiles, increased, regardless of mediastinal shift. Septal collagen fibers increased up to 2.7-fold, suggesting a greater need for structural support. Compared with previous cohorts following less-extensive resection, tissue volume and gas-exchange surface areas increased significantly only in the infracardiac lobe following 42% resection, exceeded two- to threefold in all lobes following 58% resection, and then exhibited diminished gains following similar to 70% resection. In contrast, alveolar-capillary formation increased with incremental resection without reaching an upper limit. Overall structural regrowth was most vigorous and uniform following 58% resection. The diminishment of gains in tissue growth, following similar to 70% resection, could reflect excessive or maldistributed mechanical stress that threatens septal integrity. Results also suggest additional independent stimuli of alveolar-capillary formation, possibly related to the postresection augmentation of regional perfusion.