One of the factors driving the expansion of the road transport network is the need to meet the raw material requirements faster, to ship the goods to the market and to ensure the goods are delivered to the consumers, as a result of the industrial revolution which in turn gave rise to a rapid development era. The development and transformation processes during the 18th and 19th centuries triggered inter-industry relationships and brought about a socio-economic and cultural transformation along with a change which came with its own spatial consequences. A variety of ecological problems and risks increasingly emerging are one of the, if not the foremost, indirect consequences in terms the environment. It is intended to present the results of a study on establishing checklists and valuation matrices which give a better insight on the ecological effects of transportation investment projects. A method involving identification and analysis of the problem and determining the risk level based on the ERA (Ecological risk assessment) guidelines issued by USEPA (United Nations Environmental Protection Agency) in 1998 was employed in the study.