SYNTHESIS AND IN VITRO EVALUATION OF GENISTEIN-LOADED NANOPARTICLES FOR BREAST CANCER TREATMENT


Bakar G. M., Tatar C., Erdemir A.

2. INTERNATIONAL DiCLE SCIENTIFIC STUDIES AND INNOVATION CONGRESS, Diyarbakır, Turkey, 28 - 29 May 2022, pp.180

  • Publication Type: Conference Paper / Summary Text
  • City: Diyarbakır
  • Country: Turkey
  • Page Numbers: pp.180
  • Yıldız Technical University Affiliated: Yes

Abstract

Breast cancer is the most common type of cancer in women. Although there are various conventional methods to treat breast cancer, these methods have many harmful side effects. For these reasons, more natural methods are sought for cancer treatment such as the use of flavonoids. Flavonoids are various chemicals found naturally in fruits and vegetables and have been observed in various experiments to induce cancer. One of these flavonoids is genistein, which is found in soy products, and its cancer-reducing effects have been observed in numerous studies, especially on breast cancer. But because of its very low solubility, very high amounts of fruit are required to obtain several flavonoids to act as a medicine. With this project, genistein was encapsulated into poly lactic-co-glycolic acid (PLGA) nanoparticles to increase the solubility of genistein. Genistein-loaded PLGA nanoparticles (GenNP) are produced by a single emulsion (o/w) solvent evaporation method. Zetasizer Nano ZS was used to determine the particle size, size distribution index (PDI), and zeta potential values of the nanoparticles generated. Fourier transform infrared (FT-IR) spectroscopy was used to examine functional groups on the surface of nanoparticles. Following physicochemical evaluation, the mean size of GenNP was determined as 144,4±6,28 nm, the PDI value was 0,194±0,014, and the zeta potential was -31,466±1,05 mV. The produced nanoparticle encapsulation efficiency was found as 23,3%, and drug loading capacity was determined as 13,86%. Furthermore, according to FT-IR spectroscopy, the nanoparticles formed carry PLGA properties and encapsulation of genistein is performed effectively. With this method, a higher apoptosis rate and lower cytotoxicity than classical methods are expected, which will enable it to be more effective in cancer treatment because of increasing solubility.