© 2022 Hydrogen Energy Publications LLCIncreasing the biohydrogen production efficiency through the dark fermentation process has become the biggest challenge in recent years. We aim to enhance biohydrogen production yield by adding nano-sized iron oxide doped metal oxides prepared by the wet impregnation method. Biohydrogen production in the presence of additives was evaluated by the screening of (i) type (Fe2O3@Al2O3, Fe2O3@ZrO2, and Fe2O3@TiO2) and (ii) concentration (0–200 mg/L) of additives. During the screening of additive type, approximately 14 wt% nano-sized (6 nm) hematite (Fe2O3) doped Al2O3 showed the highest improvement for biohydrogen production yield among all additives. Batch experiments conducted through dark fermentation demonstrated that 50 mg/L Fe2O3@Al2O3 addition caused an acceleration in biohydrogen production by 34% and an increment in yield by 15%, despite even low concentrations Al2O3 addition inhibited the process.