ASIAN JOURNAL OF ANIMAL AND VETERINARY ADVANCES, vol.7, no.4, pp.356-360, 2012 (SCI-Expanded)
Article / Article
ASIAN JOURNAL OF ANIMAL AND VETERINARY ADVANCES
Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA)
E1 enzyme, heat stress, RT-PCR, Carassius auratus, CYCLE MUTANT TS85, EARLY PROTEIN ICP4, PROTEASOME SYSTEM, VIRUS TYPE-1, E1, EXPRESSION, GENE, CELLS, COMPLEMENTATION, GAMETOGENESIS
Yıldız Technical University Affiliated:
The stress effect of two different temperatures on mRNA level of ubiquitin-activating enzyme (E1) in ovarian tissue of goldfish was compared. For this purpose, PCR product of 600 bp of E1 was amplified in fish was kept at two different stress temperatures (2 h at+7 degrees C and 2 h at+14 degrees C). At the end of study, there was no any difference between the extent of RT-PCR bands of E1 at 2 h+7 degrees C heat stress and the control group, there was not RT-PCR band of E1 at 2 h+14 degrees C heat stress.