Optimization of callus culture for rosmarinic acid production from Salvia officinalis: Scale-up scenarios and economic evaluation
Biochemical Engineering Journal, cilt.237, 2027 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 237
- Basım Tarihi: 2027
- Doi Numarası: 10.1016/j.bej.2026.110388
- Dergi Adı: Biochemical Engineering Journal
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chimica, Compendex, EMBASE, INSPEC, Academic Search Ultimate (EBSCO), Engineering Source (EBSCO)
- Anahtar Kelimeler: Callus, Dicamba, Rosmarinic acid, Salvia officinalis, Techno-economic analysis
- Yıldız Teknik Üniversitesi Adresli: Evet
Özet
This study explores the effects of dicamba and its combination with kinetin on callus biomass and rosmarinic acid (RA) accumulation in Salvia officinalis and is supported by a techno-economic assessment of the feasibility of large-scale production. Dicamba at 2.0 mg/L significantly increased callus biomass (2.98 g fresh weight), although it was not significantly different from its combination with kinetin, and no significant variation in callus diameter was observed among treatments. RA levels varied with plant growth regulator composition, with the highest value (45.65 mg/g DW) obtained in MS medium supplemented with 2.0 mg/L dicamba and 0.5 mg/L kinetin. These findings suggest a differential effect of dicamba on biomass formation and metabolite accumulation, with co-application of kinetin associated with higher RA levels. Techno-economic analysis, based on process modeling of capital and operating costs, further supports the feasibility of this system as a controlled and scalable platform for RA production. Collectively, these results suggest that dicamba, particularly when combined with kinetin, may be a promising approach to enhancing RA production in S. officinalis callus cultures, with potential implications for the future development of more efficient and sustainable RA production processes.