MUTATION ANALYSIS OF NUCLEOPROTEIN, VP35, VP30, AND L PROTEINS OF THE MARBURG VIRUS AND IN SILICO EVALUATION OF THEIR POTENTIAL AS VACCINE CANDIDATES

Tarhan B., Öztürk S., Aktaş E., Özdemir Özgentürk N.

ARCENG III. INTERNATIONAL SCIENTIFIC RESEARCH CONGRESS, İstanbul, Türkiye, 1 - 02 Şubat 2025, ss.161-168

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Basıldığı Şehir: İstanbul
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.161-168
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

Millions of people have lost their lives in the SARS-CoV pandemic in recent years. During the pandemic, vaccines played a critical role in both controlling the pandemic and accelerating the return to normal life. This situation has accelerated the development of vaccines against other viruses and once again demonstrated the importance of vaccine studies. One of the aims of our current study is to examine the Nucleoprotein, VP35, VP30, and Large Protein (L), which are the main structural proteins involved in the replication of the genetic material of the Marburg virus, which has pandemic potential according to the World Health Organization, for potential vaccine study. Marburg virus is a pathogen known for its high mortality rates and can cause serious epidemics. Therefore, the development of an effective vaccine against the virus is of great importance. For this purpose, important parameters such as physicochemical and biochemical properties of proteins, 2D structures, solubility, transmembrane helices, localization, signal peptides, and antigenicity-allergenicity were evaluated using various bioinformatics analysis tools. Studies in the SARS-CoV pandemic have shown that virus mutations can alter vaccine efficacy. Therefore, another goal of our study was to identify the mutation sites of the proteins analyzed. Mutation of viruses can reduce or completely eliminate the efficacy of vaccines. For this purpose, mutations of vaccine candidate proteins were documented in detail using the MEGAX application. The results obtained are expected to be an important resource not only for the Marburg virus but also for studies on other members of the filovirus family, including the Marburg virus, such as the Ebola virus. The filovirus includes many viruses known for their high mortality rates and epidemic potential. The findings of this study may shed light on the development of effective vaccines not only against Mar