Non-Invasive Holographic Sensor System for Measuring Cell Stiffness


Işik Y., Abdioğlu H. B.

Deney Föyü, ss.1-10, 2024

  • Yayın Türü: Diğer Yayınlar / Deney Föyü
  • Basım Tarihi: 2024
  • Sayfa Sayıları: ss.1-10
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

Advancements in cellular biomechanics have laid the foundation for novel diagnostic tools, yet

measuringcellstiffnessremainschallengingduetothedisruptioncausedbytraditionalmethods,such

as atomic force microscopy (AFM) or optical stretching. Our study introduces a new non-invasive

holographic sensor system that overcomes these challenges by using real-time holographic imaging

and acoustic stimulation. This sensor employs an off-axis Mach-Zehnder interferometer with bulk

acoustic waves to capture and analyze the mechanical responses of cells. Unlike AFM and optical

techniquesthatareinvasiveorrequirespecializedequipment,oursensorprovideslabel-freeandhigh-

throughput measurements. It also preserves cellular integrity, making it ideal forsensitive diagnostic

and research applications. Preliminary tests on polyacrylamide beads, which mimic cell stiffness,

demonstrate high precision and consistency across measurements, underscoring its potential utility

in early cancer detection, disease progression monitoring, and mechanobiological research.