Akademik Veri Yönetim Sistemi
International Congress on Oil and Protein Crops, Antalya, Türkiye, 2 - 04 Kasım 2023, ss.120
In this study, we focused on optimizing the parameters for extracting proteins from milk thistle
seeds, with a primary emphasis on enhancing purity and yield. Additionally, we conducted a
comprehensive characterization of the proteins isolated from these seeds. The ideal conditions
for achieving high protein purity were identified as follows: a pH level of 9.47, a temperature
of 30°C, and an extraction duration of 180 minutes. Conversely, for maximizing protein yield,
the optimal conditions were determined to be a pH level of 12, a temperature of 50°C, and an
extraction duration of 167 minutes. The Oil Absorption Capacity (OAC), emulsifying
properties, foaming capacity, and stability of both Milk Thistle Protein (MTP) and Milk Thistle
Yield (MTY) were found to be comperatable with several other seed proteins documented in
the literature. Notably, both proteins exhibited their highest solubility at a pH of 11, while their
zeta potentials approached zero at pH 4, indicating their proximity to the isoelectric point. It is
noteworthy that MTP and MTY exhibited relatively lower antioxidant capabilities compared to
other protein isolates or concentrates. Furthermore, these proteins were found to have a high
concentration of β sheets, suggesting their potential for excellent thermalstability and resistance
to digestion. Consequently, milk thistle proteins appear to hold promise for various
applications, given their unique characteristics.