In the present study, we prepared hydrogel containing synthesized TiO2 nanoparticles to investigate the antibacterial properties of these hydrogels. TiO2 nanoparticles were synthesized by a sonochemical method. The TiO2 containing hydrogel was characterized using thermogravimetry (TG) and Fourier transform infrared spectroscopy (FTIR) techniques. The antibacterial activities of the hydrogels against Escherichia coli were measured by the airborne testing and modified Kirby Bauer disk diffusion method. Finally, we demonstrated the presence of antibacterial activity in the hydrogel containing synthesized TiO2 nanoparticles.