In vitro crown galls induced by Agrobacterium tumefaciens strain A281 (pTiBo542) in Trigonella foenum-graecum


Khawar K., Gulbitti-Onarici S., Cocu S., Erisen S. , Sancak C., Ozcan S.

BIOLOGIA PLANTARUM, cilt.48, ss.441-444, 2004 (SCI İndekslerine Giren Dergi) identifier identifier

Özet

Transformation of fenugreek ( Trigonella foenumgraecum) was carried out with A281 oncogenic strain of Agrobacterium tumefaciens using root, cotyledon and hypocotyl explants excised from 1-week-old seedlings, which showed that the plant was highly susceptible to transformation. Tumors ( calli) were selected on 50 mg dm(-3) kanamycin. They were analyzed for beta-glucuronidase (GUS) expression. Presence of uidA (gus) gene, was confirmed by polymerase chain reaction (PCR) amplification.