In vitro crown galls induced by Agrobacterium tumefaciens strain A281 (pTiBo542) in Trigonella foenum-graecum

Khawar K., Gulbitti-Onarici S., Cocu S., Erisen S., Sancak C., Ozcan S.

BIOLOGIA PLANTARUM, cilt.48, sa.3, ss.441-444, 2004 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 48 Sayı: 3
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1023/b:biop.0000041100.94688.2d
  • Dergi Adı: BIOLOGIA PLANTARUM
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.441-444
  • Anahtar Kelimeler: transformation, kanamycin resistance, GUS analysis, PCR, EXPRESSION, LOCALIZATION, GROWTH, PLANTS, CELLS
  • Yıldız Teknik Üniversitesi Adresli: Hayır

Özet

Transformation of fenugreek ( Trigonella foenumgraecum) was carried out with A281 oncogenic strain of Agrobacterium tumefaciens using root, cotyledon and hypocotyl explants excised from 1-week-old seedlings, which showed that the plant was highly susceptible to transformation. Tumors ( calli) were selected on 50 mg dm(-3) kanamycin. They were analyzed for beta-glucuronidase (GUS) expression. Presence of uidA (gus) gene, was confirmed by polymerase chain reaction (PCR) amplification.