Electron spin resonance studies on cobalt carbonic anhydrase-substrate complexes


Nuhoglu C., Demir N., NALBANTOĞLU B. , Küfrevioğlu Ö. İ. , Yoğurtçu Y. K. , Ozdemir H., et al.

TURKISH JOURNAL OF CHEMISTRY, cilt.21, ss.134-138, 1997 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 21 Konu: 2
  • Basım Tarihi: 1997
  • Dergi Adı: TURKISH JOURNAL OF CHEMISTRY
  • Sayfa Sayısı: ss.134-138

Özet

The native zinc atom of bovine erythrocyte carbonic anhydrase purified by affinity choromatography was removed by dialysis against pyridine 2,6-dicarboxylic acid. Cobalt carbonic anhydrase was prepared from the zinc-free apoenzyme. The binding conditions of CO2 and p-nitrophenylacetate to cobalt carbonic anhydrase were investigated by electron spin resonance at different pH levels.