Electron spin resonance studies on cobalt carbonic anhydrase-substrate complexes


Nuhoglu Ç. , Demir N., NALBANTOĞLU B. , Küfrevioğlu Ö. İ. , Yoğurtçu Y. K. , Ozdemir H., ...More

TURKISH JOURNAL OF CHEMISTRY, vol.21, no.2, pp.134-138, 1997 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 2
  • Publication Date: 1997
  • Title of Journal : TURKISH JOURNAL OF CHEMISTRY
  • Page Numbers: pp.134-138

Abstract

The native zinc atom of bovine erythrocyte carbonic anhydrase purified by affinity choromatography was removed by dialysis against pyridine 2,6-dicarboxylic acid. Cobalt carbonic anhydrase was prepared from the zinc-free apoenzyme. The binding conditions of CO2 and p-nitrophenylacetate to cobalt carbonic anhydrase were investigated by electron spin resonance at different pH levels.