ALLEVIATION OF COPPER STRESS WITH HOMOBRASSINOSTEROID IN GERMINATING SUNFLOWER ROOTS

Burun K. B., Marakli S., Gozukirmizi N.

FRESENIUS ENVIRONMENTAL BULLETIN, vol.25, no.11, pp.4872-4878, 2016 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 11
  • Publication Date: 2016
  • Journal Name: FRESENIUS ENVIRONMENTAL BULLETIN
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.4872-4878
  • Keywords: Helianthus annuus L., heavy metal, plant hormone, root growth, antioxidant enzyme activity, INDUCED OXIDATIVE STRESS, ENZYME-ACTIVITIES, ANTIOXIDANT, RESPONSES, BRASSINOSTEROIDS, PLANTS, SALT, PHOTOSYNTHESIS, METABOLISM, SYSTEM
  • Yıldız Technical University Affiliated: No

Abstract

We investigated potential alleviation effects of homobrassinosteroid (HBR) hormone on sunflower (Helianthus annuus L. cv. TR003) grown under copper (Cu) stress conditions. Seeds were grown under Cu (30 mu M and 40 mu M) and also HBR (2 mu M) treatments to analyse primary root lengths, protein contents, antioxidant system enzymes' activities (superoxide dismutase-SOD and catalase-CAT) and expressions of these enzymes at 48 h and 72 h. When compared to control, Cu applications decreased primary root lengths. On the other hand, HBR application increased primary root lengths of Cu-stressed samples at 48 h and 72 h. Depending on concentration and timing, protein contents and enzyme activities showed varying results in Cu-treated and Cu+HBR-applied samples. In addition to enzyme activities, SOD and CAT expression profiles were also investigated by RT-PCR. Expected band sizes for SOD (309 bp) and CAT (248 bp) were observed in all samples. These results indicate a positive role of HBR on Cu-stressed sunflower for agricultural applications.