In the present study, effect of Baikal EM1, a product of EM technology, on the root collar diameter and height growth of 1+0 aged seedlings of Anatolian black pine [Pinus nigra Arnold. subsp. pallasiana Lamb. (Holmboe)] growing in Kozcagiz Temporary Nursery was investigated. Seedlings were treated with three different concentrations of Baikal EM1 solution from seed dibbling. Related morphologic parameters were measured in four treatment types at the end of vegetation season. Statistical comparison of morphologic data for treatments was performed using ANOVA test while the treatments were categorised using Duncan test. Compared to control, mean root collar diameter growth was found to be 20% higher in seedlings in T1 treatment type (Baikal EM1 concentration of 1:1000) and nearly 31% more in T2 and T3 treatment type while height growth was 22% more in T1 treatment and 34% in T2 and T3 treatment. Difference between treatment groups for root collar diameter and height growth was found to be statistically significant in from the results of ANOVA test (p<0.05). According to the results of Duncan test, T2 and T3 treatment categories where Baikal EM1 was used in the concentration rates of 1:500 and 1:100 respectively, took place in the same group considering both morphological values. It was suggested form the result of the study that Baikal EM1 should be used in the concentration of 1:500 for the 1+0 aged seedlings of Anatolian black pine originating from Kumluca and growing under the conditions of Kozcagiz Temporary Nursery. Findings of the study were in convenience with literature and Baikal EM1, a product of EM technology, was observed to contribute positively to the root collar diameter growth of the seedlings of Anatolian black pine. It is thought that Baikal EM1 can be used to obtain quality black pine seedlings in nurseries by developing root collar and plant height. It was also suggested that effect of Baikal EM1 on the growth of seedlings in different growing mediums of nurseries should be investigated by taking different species including forest and fruit trees at various ages.