Sono-photodynamic therapy (SPDT), which is used in the treatment of cancer cells and is advantageous in therapeutic results, has been studied rarely academically as it is a new method. That's why a novel multicomponent system, ZnPc-[Ru(bpy)(2)(phen)](4), as a photosensitizer (Ps) was synthesized by the condensation reaction of the symmetrical zinc phthalocyanine substituted with four 4-(4-formylphenoxy) groups at peripheral positions and ruthenium (II) bis(bipyridine)-phenanthroline complexes including amine group. The compounds were characterized by elemental analysis, FT-IR, H-1 NMR, UV-Vis and MALDI-TOF MS spectral data and analyzed for photophysico-chemical properties. Singlet oxygen quantum yields of the photosensitizer were calculated using photochemical and sono-photochemical methods. When singlet oxygen yields were compared, higher efficiencies were achieved in singlet oxygen production by the sono-photochemical method ( Phi(delta)= 0.72 in DMSO, 0.66 in DMF) compared to the photochemical method (Phi(delta) = 0.45 in DMSO, 0.43 in DMF). This work will lead to the synthesis of new sensitizers with high potential for use in SPDT. (C)& nbsp;2022 Elsevier B.V. All rights reserved.