A novel graphene oxide-based fluorescence method for detection of urine glycosaminoglycans


Bakır Köseoğlu C., Dede S., Karakuş E., Erdoğmuş A., Keskin B., Önal H.

Biotechnology and Applied Biotechemistry, vol.1, no.1, pp.1-10, 2024 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 1 Issue: 1
  • Publication Date: 2024
  • Journal Name: Biotechnology and Applied Biotechemistry
  • Page Numbers: pp.1-10
  • Yıldız Technical University Affiliated: Yes

Abstract

Glycosaminoglycans (GAGs) serve as a biomarker for mucopolysaccharidoses disease. In this study, a novel fluorometric method was developed to measure total GAGs in urine. Graphene oxide (GO) and rhodamine B (RhB), a cationic flu- orescent dye, were employed in the development of the method. RhB attaches to the GO surface via electrostatic attraction, leading to the quenching of its fluores- cence upon the establishment of the RhB–GO complex. However, the presence of GAGs prompts a resurgence of intense fluorescence. The linear range of the method is between 5.00 and 70.00 mg/L. The total GAG levels of urine samples analyzed using the method agree with the results of the biochemistry analysis laboratory (65.85 and 79.18 mg/L; 73.30 ± 1.76 and 72.21 ± 2.21). The method is simple, accurate, and sensitive and may be used for both first-step diagnosis of the mucopolysaccharidoses and detection of individual GAGs for studies of GAG-related research and other biological applications.

KEYWORDS

fluorescence-based sensor, graphene oxide, mucopolysaccharidosis, rhodamine B