Effects of different cytoplasmic chaperones on outer membrane solubility in Escherichia coli; rOMP25 modeling

Trabzonlu K., ÖZBEK T.

Sigma Journal of Engineering and Natural Sciences, cilt.41, sa.5, ss.907-915, 2023 (ESCI) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 5
  • Basım Tarihi: 2023
  • Doi Numarası: 10.14744/sigma.2022.00106
  • Dergi Adı: Sigma Journal of Engineering and Natural Sciences
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, Academic Search Premier, Directory of Open Access Journals
  • Sayfa Sayıları: ss.907-915
  • Anahtar Kelimeler: Brucellosis, Inclusion Body, Molecular Chaperones, Protein Expression, Purification, rOmp25
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

In this study, for the first time in the literature, is investigated the effects of different chaperones on outer transmembrane membrane protein solubility by modeling the expression of the recombinant Omp25 outer membrane protein, which is one of the main antigens against brucellosis and known to form inclusion bodies in the cytoplasm.