Effects of different cytoplasmic chaperones on outer membrane solubility in Escherichia coli; rOMP25 modeling

Trabzonlu K., ÖZBEK T.

Sigma Journal of Engineering and Natural Sciences, vol.41, no.5, pp.907-915, 2023 (ESCI) identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 5
  • Publication Date: 2023
  • Doi Number: 10.14744/sigma.2022.00106
  • Journal Name: Sigma Journal of Engineering and Natural Sciences
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, Academic Search Premier, Directory of Open Access Journals
  • Page Numbers: pp.907-915
  • Keywords: Brucellosis, Inclusion Body, Molecular Chaperones, Protein Expression, Purification, rOmp25
  • Yıldız Technical University Affiliated: Yes

Abstract

In this study, for the first time in the literature, is investigated the effects of different chaperones on outer transmembrane membrane protein solubility by modeling the expression of the recombinant Omp25 outer membrane protein, which is one of the main antigens against brucellosis and known to form inclusion bodies in the cytoplasm.