Investigation of heavy metal effects on immobilized paraoxanase by glutaraldehyde


Bilen Ç. , Beyaztas S., Arslan O., Guler O. O.

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, vol.28, pp.440-446, 2013 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 28
  • Publication Date: 2013
  • Doi Number: 10.3109/14756366.2011.647007
  • Title of Journal : JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
  • Page Numbers: pp.440-446

Abstract

Serum paraoxonase 1 (PON1) was purified from bovine serum using hydrophobic interaction chromotography on Sepharose 4B-coupled L-tyrosine 1-naphthylamine gel, and monitored by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Paraoxonase enzyme was immobilized using different ratios of glutaraldehyde and the maximum activity was observed with 7% glutaraldehyde. The effects of inhibition by Mn+2, Co+2 and Cu+2 heavy metals on the immobilized and free enzyme activities were studied. At the optimum pH and temperature, the K-m and V-max kinetic values for bovine serum paraoxonase and immobilized paraoxonase towards paraoxon substrate were determined as 0.296 x 10(-3) M & 37.04 EU vs. 0.727-10(-3) M & 36.36 EU, respectively.