Investigation of heavy metal effects on immobilized paraoxanase by glutaraldehyde


Bilen Ç. , Beyaztas S., Arslan O., Guler O. O.

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, cilt.28, ss.440-446, 2013 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28
  • Basım Tarihi: 2013
  • Doi Numarası: 10.3109/14756366.2011.647007
  • Dergi Adı: JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
  • Sayfa Sayıları: ss.440-446

Özet

Serum paraoxonase 1 (PON1) was purified from bovine serum using hydrophobic interaction chromotography on Sepharose 4B-coupled L-tyrosine 1-naphthylamine gel, and monitored by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Paraoxonase enzyme was immobilized using different ratios of glutaraldehyde and the maximum activity was observed with 7% glutaraldehyde. The effects of inhibition by Mn+2, Co+2 and Cu+2 heavy metals on the immobilized and free enzyme activities were studied. At the optimum pH and temperature, the K-m and V-max kinetic values for bovine serum paraoxonase and immobilized paraoxonase towards paraoxon substrate were determined as 0.296 x 10(-3) M & 37.04 EU vs. 0.727-10(-3) M & 36.36 EU, respectively.