Akademik Veri Yönetim Sistemi
4th International Environmental Chemistry Congress, Antalya, Türkiye, 31 Ekim - 02 Kasım 2022, ss.59-60
Garlic (Allium sativum) is a universal plant of the Liliaceae family that has been used for centuries for its biological and medicinal properties. These vegetables contain high amounts of organosulphur compounds (OSCs)1,2,3 . This study aimed to develop a simple, sensitive, and accurate measuring method that uses reversed phase-high performance liquid chromatography (RP-HPLC) to determine the concentrations of selected organosulfur compounds (OSCs) in garlic bulbs. In order to extract oil-soluble and water-soluble OSCs from garlic matrix, acetonitrile and deionized water were used. The OSCs were separated on a Phenomenex C18 column (250 mm, 4 mm, and 5 mm) and monitored by a UV detector at a wavelength of 220 nm. The mobile phase used in isocratic elution was 0.10 M trifluoroacetic acid (TFA) in 85% acetonitrile (ACN) and 0.10 M TFA in distilled water (DW) (90:10, % v/v). In optimal experimental conditions, the limit of detection (LOD) was calculated in the range of 0.09 - 0.17 mg/kg. Dialyl sulfide (DAS), diallyl disulfide (DADS), and diallyl trisulfide (DATS) were detected in garlic samples under the developed instrumental system in concentration ranges of 8.0-32.5 mg/kg, 20.4-67.3 mg/kg, and 60.7-356.6 mg/kg, respectively. The accuracy and applicability of this method were confirmed by spiked samples experiments conducted on garlic samples. For the garlic samples extracted in deionized water, recovery values ranged from 39.0 to 90.9%. This strategy is appropriate for the determination of OSCs because it is precise, accurate, reliable, and time-effective.