Nanofibers fabricated by the electrospinning process demonstrate excellent surface area, high porosity, phase purity, and good chemical composition. In this study, we fabricated pure poly vinyl alcohol (PVA) nanofiber and glycyl-L-histidyl-L-lysine (GHK) embedded PVA nanofibers by using the electrospinning technique and characterized the fibers using relevant methods. Scanning electron micro-scopic images demonstrated that the average dimensions of the fabricated PVA fibers at 20 and 25 wt% were 100 and 200 nm, respectively, and they possessed equivalent diameters. In contrast, GHK embedded PVA nanofiber dimensions varied between 50 and 250 nm. The Fourier transform-infrared spectrophotometry results revealed the bonds related to PVA and GHK-PVA conjugation. In addition cell culture studies showed that the nanofibers were non-toxic. Growth-modulating peptide (GHK) embedded poly vinyl alcohol was electrospun to yield nano fibers that can be used for wound dressing applications.