Frontal chromatography of proteins: The effect on column performance of the restricted diffusion of molecules in porous chromatographic adsorbents

LIAPIS A., Sadikoglu H., CROSSER O.

JOURNAL OF CHROMATOGRAPHY A, vol.828, pp.345-356, 1998 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 828
  • Publication Date: 1998
  • Doi Number: 10.1016/s0021-9673(98)00642-6
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.345-356
  • Yıldız Technical University Affiliated: No


A theoretical formulation is presented that can be used to describe the dynamic behavior of frontal chromatography of proteins in columns packed with adsorbent particles in which restricted pore diffusion of the adsorbate molecules occurs. The results of this work clearly indicate that the time for breakthrough and the effective utilization of the adsorptive capacity of the chromatographic particles increase as (a) the size of the adsorbate and/or ligand (active site) molecule decreases, (b) the pore connectivity, n(T), of the porous network of the adsorbent particles increases, and (c) the column length, L, increases. (C) 1998 Elsevier Science B.V. All rights reserved.