Determination of genotoxic effects of boron and zinc on Zea mays using protein and random amplification of polymorphic DNA analyses

Erturk F. A., Nardemir G., Hilal A., ARSLAN YÜKSEL E., AĞAR G.

Toxicology and Industrial Health, vol.31, no.11, pp.1015-1023, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 11
  • Publication Date: 2015
  • Doi Number: 10.1177/0748233713485888
  • Journal Name: Toxicology and Industrial Health
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1015-1023
  • Keywords: Boron, genomic template stability, RAPD-PCR, total soluble protein, zinc
  • Yıldız Technical University Affiliated: No

Abstract

In this research, we aimed to determine genotoxic effects of boron (B) and zinc (Zn) on Zea mays by using total soluble protein content and random amplification of polymorphic DNA (RAPD) analyses. For the RAPD analysis, 16 RAPD primers were found to produce unique polymorphic band profiles on treated maize seedlings. With increased Zn and B concentrations, increased polymorphism rate was observed, while genomic template stability and total soluble protein content decreased. The treatment with Zn was more effective than that of B groups on the levels of total proteins. The obtained results from this study revealed that the total soluble protein levels and RAPD profiles were performed as endpoints of genotoxicity and these analyses can offer useful biomarker assays for the evaluation of genotoxic effects on Zn and B polluted plants. © 2013, SAGE Publications. All rights reserved.