Determination of genotoxic effects of boron and zinc on Zea mays using protein and random amplification of polymorphic DNA analyses

Erturk F. A., Nardemir G., Hilal A., ARSLAN YÜKSEL E., AĞAR G.

Toxicology and Industrial Health, cilt.31, sa.11, ss.1015-1023, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 11
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1177/0748233713485888
  • Dergi Adı: Toxicology and Industrial Health
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1015-1023
  • Anahtar Kelimeler: Boron, genomic template stability, RAPD-PCR, total soluble protein, zinc
  • Yıldız Teknik Üniversitesi Adresli: Hayır

Özet

In this research, we aimed to determine genotoxic effects of boron (B) and zinc (Zn) on Zea mays by using total soluble protein content and random amplification of polymorphic DNA (RAPD) analyses. For the RAPD analysis, 16 RAPD primers were found to produce unique polymorphic band profiles on treated maize seedlings. With increased Zn and B concentrations, increased polymorphism rate was observed, while genomic template stability and total soluble protein content decreased. The treatment with Zn was more effective than that of B groups on the levels of total proteins. The obtained results from this study revealed that the total soluble protein levels and RAPD profiles were performed as endpoints of genotoxicity and these analyses can offer useful biomarker assays for the evaluation of genotoxic effects on Zn and B polluted plants. © 2013, SAGE Publications. All rights reserved.