Polyphasic classification of nonomuraea strains isolated from the karakum desert and description of nonomuraea deserti sp. Nov., Nonomuraea diastatica sp. nov., nonomuraea longispora sp. nov. and nonomuraea mesophila sp. nov

Saygin H., Nouioui I., Ay H., Guven K., ÇETİN D., Klenk H., ...More

International Journal of Systematic and Evolutionary Microbiology, vol.70, no.1, pp.636-647, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 70 Issue: 1
  • Publication Date: 2020
  • Doi Number: 10.1099/ijsem.0.003808
  • Journal Name: International Journal of Systematic and Evolutionary Microbiology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.636-647
  • Keywords: Actinobacteria, genomics, Nonomuraea, phylogenomic analyses, Karakum Desert
  • Yıldız Technical University Affiliated: No

Abstract

Five actinobacteria isolates, KC201T, KC401, KC310T, KC712T and 6K102T, were recovered from the Karakum Desert during an investigation of novel actinobacteria with biotechnological potential. A polyphasic approach confirmed the affiliation of the strains to the genus Nonomuraea. The strains showed chemotaxonomic and morphological properties consistent with their classification in the genus Nonomuraea. Furthermore, these strains clearly distinguished and formed well supperted clades in phylogenetic and phylogenomic trees. Low ANI and dDDH values and distinguishing phenotypic properties between isolates KC201T, KC310T, KC712T and 6K102T showed that these strains belonged to novel Nonomuraea species, the names proposed for these taxa are Nonomuraea deserti sp. nov., Nonomuraea diastatica sp. nov., Nonomuraea longispora sp. nov. and Nonomuraea mesophila sp. nov., with the type strains KC310T (=CGMCC 4.7331T =DSM 102919T =KCTC 39774T), KC712T (=CGMCC 4.7334T =DSM 102925T =KCTC 39776T), KC201T (=CGMCC 4.7339T =DSM 102917T =KCTC 39781T) and 6K102T (=CGMCC 4.7541T =JCM 32916T), respectively.