Noise pollution - one of the major environmental problems, affects people both physiologically and psychologically. Noise measurements must be carried out regularly to overcome the noise problem. The study in this paper was conducted to determine the noise level in different parts of an education and research hospital in Istanbul. Noise levels were measured at the main entrance, pediatric department, emergency room entrance, audiology clinic, nuclear medicine cardiology clinic, eye clinic, and emergency service corridor of the hospital. Measurements were taken on Monday, Tuesday, Wednesday, Thursday, and Friday, every hour from 08:00 in the morning to 17:00 in the afternoon. As a result, average noise levels were found between 52 dB and 57 dB in different parts of the hospital. The results were compared with similar studies and recommendation in WHO guidelines.