One-Step Electrochemical Fabrication of Lithium-Intercalated Pencil Graphite Electrode (PGE) for the Differential Pulse Voltammetric (DPV) Determination of l-Tyrosine


Serbest Z., Gördük Ö., Şahin Y.

ANALYTICAL LETTERS, vol.56, no.7, pp.1031-1047, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 56 Issue: 7
  • Publication Date: 2023
  • Doi Number: 10.1080/00032719.2022.2117370
  • Journal Name: ANALYTICAL LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.1031-1047
  • Keywords: Differential pulse voltammetry (DPV), electrochemically treated pencil graphite electrode (EPGE), electrochemical treatment, l-tyrosine, GLASSY-CARBON ELECTRODE, GRAPHENE OXIDE, ASCORBIC-ACID, L-TRYPTOPHAN, IMPEDANCE SPECTROSCOPY, MASS-SPECTROMETRY, URIC-ACID, PHENYLALANINE, NANOPARTICLES, DOPAMINE
  • Yıldız Technical University Affiliated: Yes

Abstract

Tyrosine (l-Tyr), which is found in basic foods, is a nonessential amino acid and a metabolite of phenylalanine, has many roles in human metabolism, and is important in maintaining nutritional balance. In this study, an electrochemical method has been presented to determine low levels of l-Tyr using an electrochemically treated pencil graphite electrode (EPGE) via overoxidation of graphite surface and intercalation of Li+ ions into graphite by cyclic voltammetry in the potential range between -0.3 and 2.0 V. Characterization studies of the EPGEs were carried out using scanning electron microscopy, energy-dispersive X-ray spectroscopy, electrochemical impedance spectroscopy, Fourier transform infrared spectroscopy, atomic force microscopy, and cyclic voltammetry. The determination of l-Tyr was performed by differential pulse voltammetry, and the investigation of electrochemical properties of l-Tyr was conducted by cyclic voltammetry. The results showed that the EPGE presents high electrocatalytic performance and has a low detection limit (0.12 mu M) for determining l-Tyr with a wide linear range from 1.0 to 500 mu M. The selectivity of prepared EPGE was investigated in the presence of organic species. The determination of l-Try was performed in yoghurt and milk samples and good results were obtained with recoveries between 95.7% and 103.1%. Stability and repeatability studies further demonstrate the applicability of the sensor.