Simple Determination of Cystine in Artificial Urine and Drug Matrices Using Digital Image Colorimetry

Creative Commons License

Borahan Kustanto T., Zaman B. T., Polat Korkunç Ü., Gel M. S., Bakırdere S.

ANALYTICAL LETTERS, sa.58, ss.1-15, 2025 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1080/00032719.2025.2517118
  • Dergi Adı: ANALYTICAL LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.1-15
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

Smartphone-based digital image colorimetry was developed for thedetermination of cystine in pharmaceutical formulations and artificialurine samples. A lab-made colorimetric box was designed for captur-ing the digital images of sample solutions using a smartphone. Thedeveloped method employs a simple colorimetric method callednitroprusside test which specifically targets free -SH groups. Cystinewas reduced to cysteine before the colorimetric test was applied.The color intensity is inversely related to the concentration of theanalyte. The best linearity was obtained with the G color channel.Digital image processing was performed using the RGB ColorDetector mobile application to obtain analytical data. The developedmethod exhibited good linearity in the range of 29.7–00.3 mg/kg.The limits of detection and quantification were 9.7 mg/kg and32.3 mg/kg, respectively. The percent recoveries were 104.2 ± 7.8%and 108.7 ± 4.6%, which proved the accuracy and applicability of thedeveloped method