Photochemically prepared monodisperse gold nanoparticles as doxorubicin carrier and its cytotoxicity on leukemia cancer cells

Dizman H. M., Eroglu G. O., Kuruca S. E., ARSU N.

APPLIED NANOSCIENCE, cilt.11, sa.1, ss.309-320, 2021 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 1
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s13204-020-01589-3
  • Dergi Adı: APPLIED NANOSCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, Compendex
  • Sayfa Sayıları: ss.309-320
  • Anahtar Kelimeler: Photochemistry, Gold nanoparticles, Thioxanthone-disulfide, Drug delivery, Anticancer drug, FREE-RADICAL POLYMERIZATION, PHOTOINITIATOR, CHEMOTHERAPY, PARTICLES, MATRIX, TUMOR
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

In this study, a new thioxanthone derivative called 3,3 '-disulfanediylbis(1-hydroxy-9H-thioxanthen-9-one) was utilized in situ for the photochemical synthesis of monodisperse gold nanoparticles, and simple characterization was achieved by formation of a typical surface plasmon resonance band of gold nanoparticles at 535 nm accompanied by a nice pink color. Dynamic light scattering method was employed for determination of the size of the in situ-prepared gold nanoparticles and, with good polydispersity value at around 30 nm, it was in good agreement with the surface plasmon resonance band. After successful preparation of gold nanoparticles, a well-known cancer drug, doxorubicin, at different amounts was loaded onto prepared gold nanoparticles. The adsorbed and released doxorubicin amount onto the gold nanoparticles was calculated by a fluorescence spectrophotometer using a calibration curve. Doxorubicin-loaded gold nanoparticles were examined using a MTT assay for both HL-60 and K562 leukemia cancer cells and HUVEC cells, and 5 mu L doxorubicin-loaded gold nanoparticles significantly reduced cell viability. In normal HUVEC cells, 5 mu L doxorubicin-loaded gold nanoparticles were found less cytotoxic compared to doxorubicin alone. The data obtained in this study showed that gold nanoparticles loaded with 5 mu L doxorubicin are much more effective than doxorubicin used alone in cancer treatment.