Photoinduced in situ formation of clickable PEG hydrogels and their antibody conjugation

Kahveci M. Ü., Ciftci M., EVRAN S., TİMUR S., Yağcı Y.

DESIGNED MONOMERS AND POLYMERS, vol.18, no.2, pp.129-136, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 18 Issue: 2
  • Publication Date: 2015
  • Doi Number: 10.1080/15685551.2014.971392
  • Journal Name: DESIGNED MONOMERS AND POLYMERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.129-136
  • Yıldız Technical University Affiliated: Yes

Abstract

A simple approach for the preparation of a clickable poly(ethylene glycol)-based hydrogel as a polymeric support for protein immobilization via photoinitiated free radical polymerization of poly(ethylene glycol) diacrylate and propargyl acrylate is established. Bioconjugation to the obtained gels was achieved by azide-alkyne click reaction with azido-functionalized anti-immunoglobulin G (anti-IgG) and anti-His tag antibodies. Evaluation of the affinities of the PEG hydrogels to the corresponding substrates (IgG and His-tagged YFP, respectively) indicates their specific binding capability.