Urease-Dextran complexes with enhanced enzymatic activity and stability


Altıkatoğlu Yapaöz M. , Destanoglu A.

JOURNAL OF CARBOHYDRATE CHEMISTRY, vol.36, pp.325-335, 2017 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 36
  • Publication Date: 2017
  • Doi Number: 10.1080/07328303.2017.1403614
  • Title of Journal : JOURNAL OF CARBOHYDRATE CHEMISTRY
  • Page Numbers: pp.325-335

Abstract

In this study, the urease-dextran non-covalent complexes in various molar ratios were synthesized and compared to the free enzyme in terms of pH, temperature, thermal and storage stabilities. Especially, the complex with a molar ratio of n(U)/n(DA) = 40/1 showed highest thermal stability and had ca. 1.4-fold at 25 degrees C and 2.5-fold at 80 degrees C higher activity than the free enzyme. The complex showed a high catalytic activity in organic solvent. In addition, the thermal and storage stabilities of urease were improved greatly as dextran complex, which has advantages for usage in practice.