A rapid HPLC determination of C-2-C-7 aliphatic diamines by precolumn derivatization with acetylacetone in methanol-water


Asan A., Isildak I.

MIKROCHIMICA ACTA, cilt.132, ss.13-16, 1999 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 132 Konu: 1
  • Basım Tarihi: 1999
  • Doi Numarası: 10.1007/s006040050036
  • Dergi Adı: MIKROCHIMICA ACTA
  • Sayfa Sayısı: ss.13-16

Özet

A rapid reversed-phase high performance liquid chromatographic analysis for the determination of seven aliphatic diamines in water is described. Precolumn derivatization with acetylacetone is used for traces of aliphatic diamines in water-methanol (10:1 v/v) medium, The acetylacetone derivatives obtained after 15 min were extracted with an octadecylsilane functionalized silica cartridge, and then injected into the HPLC system, The HPLC system consisted of a reversed-phase column, and a spectrophotometric detector adjusted to 310nm as elution solvent a methanol-tetrahydrofuran-water (55:3:42 v/V) mixture was used. The acetylacetone derivatives of the C-2-C-7 diamines were separated with a good resolution in 23 min. The detection limits achieved for each diamine were between 0.18-0.72 ng/ml for a 100 ml water sample. The recovery of diamine derivatives from river and seawater was 88-101%, with relative standard deviations of 2.2-4.0%, and 82-93%, with relative standard deviations of 2.8-4.6%, respectively,