Research Information System
Bulgarian Chemical Communications, vol.54, no.3, pp.271-276, 2022 (Scopus)
© 2022 Bulgarian Academy of Sciences, Union of Chemists in Bulgaria.Polycomplexes containing polyelectrolyte (PE), metal and protein reveal strong immunogenecity and provide a high level of immunological protection. The solubility, composition, and stability of these polycomplexes depend on pH, metal/PE, and protein/PE ratios. Zetasizer parameters such as particle size, polydispersity, mobility and zeta potential are used to control the stability of solutions. These parameters are also very important for polyelectrolyte-protein binary conjugate and polyelectrolyte-metal-protein ternary complexes because of bioavailability, dissolution and immune toxicity of these complexes. Polyacrylic acid (PAA) is a polyanion polyelectrolyte and has been used in vaccine adjuvants due to its safety and lower toxicity. In the current study, PAA-BSA bioconjugate and PAA-Cu2+-BSA ternary biocomplex were freshly synthesized according to two different procedures in the presence of acrylic acid (AA), copper (II) ions and bovine serum albumin (BSA). The stability of PAA-BSA bioconjugate and PAA-Cu2+-BSA ternary biocomplex at different pHs was investigated by zetasizer measurements and the pH effect on the stability of PAA-BSA bioconjugate and PAA-Cu2+-BSA ternary biocomplex was determined.