Stability investigation of PAA-BSA bioconjugate and PAA-Cu2+-BSA ternary biocomplex at different pHs


Çakır B., Şakar D., Karahan M.

Bulgarian Chemical Communications, cilt.54, sa.3, ss.271-276, 2022 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 54 Sayı: 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.34049/bcc.54.3.f010
  • Dergi Adı: Bulgarian Chemical Communications
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.271-276
  • Anahtar Kelimeler: PAA-BSA bioconjugate, PAA-Cu2+-BSA ternary biocomplex, stability, zeta potential
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

© 2022 Bulgarian Academy of Sciences, Union of Chemists in Bulgaria.Polycomplexes containing polyelectrolyte (PE), metal and protein reveal strong immunogenecity and provide a high level of immunological protection. The solubility, composition, and stability of these polycomplexes depend on pH, metal/PE, and protein/PE ratios. Zetasizer parameters such as particle size, polydispersity, mobility and zeta potential are used to control the stability of solutions. These parameters are also very important for polyelectrolyte-protein binary conjugate and polyelectrolyte-metal-protein ternary complexes because of bioavailability, dissolution and immune toxicity of these complexes. Polyacrylic acid (PAA) is a polyanion polyelectrolyte and has been used in vaccine adjuvants due to its safety and lower toxicity. In the current study, PAA-BSA bioconjugate and PAA-Cu2+-BSA ternary biocomplex were freshly synthesized according to two different procedures in the presence of acrylic acid (AA), copper (II) ions and bovine serum albumin (BSA). The stability of PAA-BSA bioconjugate and PAA-Cu2+-BSA ternary biocomplex at different pHs was investigated by zetasizer measurements and the pH effect on the stability of PAA-BSA bioconjugate and PAA-Cu2+-BSA ternary biocomplex was determined.