Novel NAC-loaded poly(lactide-coglycolide acid) nanoparticles for cataract treatment: preparation, characterization, evaluation of structure, cytotoxicity, and molecular docking studies


Budama-Kilinc Y. , Cakir-Koc R. , Kecel-Gunduz S., Kokcu Y., Bicak B., Mutlu H., ...Daha Fazla

PEERJ, cilt.6, 2018 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 6
  • Basım Tarihi: 2018
  • Doi Numarası: 10.7717/peerj.4270
  • Dergi Adı: PEERJ

Özet

Background: N-acetylcarnosine (NAC), a dipeptide with powerful antioxidant properties that is extensively used as a pharmaceutical prodrug for the treatment of cataract and acute gastric disease, was investigated by molecular dynamics with the GROMACS program in order to understand the solvent effect on peptide conformation of the peptide molecule used as a component of a drug and which presents substantial information on where drug molecules bind and how they exert their effects. Besides, molecular docking simulation was performed by using the AutoDock Vina program which identify the kind of interaction between the drug and proteins. A delivery system based on poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) loaded with NAC (NAC-PLGA-NPs) for the treatment of cataract was prepared for the first time in this study in order to enhance drug bioavailability and biocompatibility. The objective of this work was to prepare and evaluate the structural formulation, characterization, and cytotoxicity studies of NAC-loaded NPs based on PLGA for cataract treatment.