In the present work the level of Indole-3-acetic acid (IAA), trans-Zeatin (t-Z) and Gibberellic acid's equivalents (GAs) were determined in dried and fresh specimen of 18 macrofungi taxa by High Performance Liquid Cromatograpy (HPLC). The phytohormones were extracted from fresh and dried samples of Cortinarius Fr. sp., Lentinus tigrinus (Bull.: Fr.) Fr., Coprinus atramentarius (Bull.: Fr.) Fr., Boletus impolitus Fr., Suillus granulatus (L.: Fr.) O. Kuntze, Leccinum scabrum (Bul.: Fr.) S. F Gray, Pleurotus ostreatus (Jacq.: Fr.) Kumm., Agaricus bernardii (Quel.) Sacc., Ptychoverpa bohemica (Krombholz) Boud., Agrocybe dura (Bolt.) Sing., Lycoperdon molle Pers.: Pers., Volvariella speciosa (Fr.: Fr.) Sing., Pleurotus eryngii (DC.: Fr.) Quel., Morchella esculenta Pers.: St. Amans, Coprinus comatus (Muell.: Fr.)Pers., Inocybe Fr. sp., Hebeloma longicaudum (Pers.: Fr.) Kumm. and Amanita gemmata (Fr.) Bertil. The highest GAs level in dry fungi was found in Ptychoverpa bohemica, 444.08 mu g/mg, whereas the lowest was found in Coprinus atramentarius, 22.13 mu g/mg. In fresh samples the highest and lowest GAs levels were found in Ptychoverpa bohemica, 148.65 mu g/mg and Coprinus atramentarius, 10.24 mu g/mg respectively.