Akademik Veri Yönetim Sistemi
9th Internatıonal "Başkent" Congress On Physıcal, Engıneerıng, And applied Sciences , Ankara, Türkiye, 20 - 23 Mayıs 2023, ss.333-336
In the evolving pharmaceutical
industry and industrial sector, microorganisms are frequently used, not only in
the fields of chemistry and food industry but also in pharmacology,
particularly for the synthesis of raw materials and active pharmaceutical
ingredients. As a result of research conducted in this field, traditional
chemical synthesis methods have been replaced by biotransformation studies
utilizing microorganisms as biological catalysts. The synthesized benzofuran
and indole derivative compounds that we will use in our study have a wide range
of biological activities. These compounds exhibit therapeutic properties such
as anti-tumor, anti-cancer, anti-Alzheimer’s, anti-inflammatory, anti-
bacterial, and anti-fungal, indicating their potential as novel drug agents.
Both compounds, with their heterocyclic structures and the electron-rich
pyrrole structure present in indole, which can form hydrogen bonds due to the
nitrogen it contains, can easily participate in chemical reactions. The indole
nucleus, which is widespread in plants, animals, and marine organisms, not only
forms the basic structure of many drugs but also represents indole derivative
biological structures such as tryptophan amino acid, the plant growth hormone
auxin, melatonin, and serotonin hormones. The Aspergillus species that we will
use in the biotransformation steps of our study are known as mold fungi, which
are among the most populous species in the fungal kingdom. It has been determined
that various Aspergillus strains can biotransform benzene and naphthalene,
nitrogen-containing heterocyclic structures, polycyclic aromatic hydrocarbons,
terpenoids, steroids, and other aliphatic and aromatic compounds. With their
enzyme complexes, they are candidate microorganisms for the synthesis of new
drug agents and/or raw materials from different compounds. In the study
conducted by Çalışkan et al. using A.niger mold, it was discovered that the
benzyl ring attached to the nitrogen atom of the indole ring was cleaved in the
biotransformation process[1]. In the first phase of our study, a benzofuran
derivative will be synthesized from the selected starting material,
5-phenyl-1,3-cyclohexadione, and biotransformation reactions will be carried
out using Aspergillus niger and Aspergillus oryzae. In the second
phase of our study, an indole derivative will be synthesized using the
benzofuran derivative, and similar biotransformation reactions will be
performed using Aspergillus niger and Aspergillus oryzae. In the
third phase, the indole compound will be selectively synthesized through
acetylation reactions. It will be subjected to biotransformation using Aspergillus
niger and Aspergillus oryzae molds. The structures of the compounds
obtained from biotransformation reactions will be elucidated using spectral
methods, followed by testing their biological activities and pharmacological
effects. Additionally, the effects of the synthesized compounds on
microorganisms will also be investigated