During the preparation of a biomaterial, one of the most important features to be considered is to obtain a material inducing cell proliferation with proper mechanical strength. We know that the mechanical strength of the artificial material to be used instead of cartilage tissue must be strong. Also, we know from the literature that the mechanical strength of BS is high among other natural biomaterials.This study includes the evaluation of the usability of bacterial cellulose (BS) as a scaffold for chondrocytes. BS was produced by Acetobacter xylinum and its usability was considered as artificial cartilage in the future, In the study, FTIR spectrophotometer was used for the chemical characterization of BS. Thermal properties of BS were determined by thermogravimetric (TG) / differential thermal analysis (DTA) measurements. A material microscope was used to analyze the surface morphology of the membrane obtained. Chondrocyte attachment on BS membrane was evaluated by MTT assay. 80.2% cell attachment was observed