© 2022 Elsevier B.V.Structural, optical and dielectric properties determining the conduction mechanism were investigated using appropriate measurement techniques to determine the suitability of low-concentrations (LC) (1000 μL, 500 μL, 250 μL and 125 μL) reactive red 45 (RR-45) dye loaded hydrogels for electrical circuit applications. The UV spectra of the all samples displayed a fluctuating behaviour depending on the concentration. It was found that the dielectric plane plots for all the samples are compatible with the Cole-Cole relaxation model and correspond to an equivalent electrical circuit (RC) representing with the impedance circuit in the Smith Chart. It was concluded that RR-45 dye loaded hydrogels could be a suitable material for electrodes used in electroencephalography and electrocardiography systems due to the decrease of the real part of complex dielectric constant and impedance values with increasing frequency. The s-parameter for the samples, which determines the conductivity mechanism, was calculated separately for different regions.