Trace analysis of amines in cheese serum with liquid chromatographic potentiometric detection by using amine-selective electrode

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Aydin R., Asan A., ATTAR A., IŞILDAK İ.

ARABIAN JOURNAL OF CHEMISTRY, vol.12, no.8, pp.4533-4540, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 8
  • Publication Date: 2019
  • Doi Number: 10.1016/j.arabjc.2016.07.006
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.4533-4540
  • Keywords: Amine-selective electrodes, Amine analysis, Potentiometric detection, High performance liquid chromatography, BIOGENIC-AMINES, ALIPHATIC-AMINES, DERIVATIZATION, DRUGS
  • Yıldız Technical University Affiliated: Yes


A trace quantification liquid chromatographic-potentiometric method for the determination of amines in cheese serum is described. An all solid-state contact polyvinyl chloride membrane amine-selective electrode was prepared and used as a detector in a low-dead volume flow through detection cell. Potentiometric performance characteristics of the proposed electrodes were then examined. A slope of 52.4 +/- 3.8 mV per decade toward amines was received at the concentration range of 1.0 x 10(-2)-8.0 x 10(-6) M (r(2) = 0.99) under dynamic conditions. Six amine compounds were separated, with a good resolution in the chromatographic system by using acetonitrile-methane sulfonic acid mobile phase gradient at the flow-rate of 1 mL min(-1) in 30 min. The potentiometric detector was successfully applied to the analysis of amines in cheese serum samples. The relative standard deviations were ranged from 2.2% to 4.0% and recoveries were ranged from 88% to 101% for the determination. The average concentrations of ethylamine, butylamine and hexylamine in cheese serum samples calculated were 6.46, 4.51 and 8.37 mu g L-1 respectively. (C) 2016 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.