Nisin Production of Lactococcus lactis N8 with Hemin-Stimulated Cell Respiration in Fed-Batch Fermentation System

Kordikanlioglu B., Simsek Ö., Saris P. E. J.

BIOTECHNOLOGY PROGRESS, vol.31, no.3, pp.678-685, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 3
  • Publication Date: 2015
  • Doi Number: 10.1002/btpr.2075
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.678-685
  • Keywords: L. lactis, nisin, respiration, hemin, fed-batch fermentation, GROWTH, KINETICS, CHITIN
  • Yıldız Technical University Affiliated: No


In this study, nisin production of Lactococcus lactis N8 was optimized by independent variables of glucose, hemin and oxygen concentrations in fed-batch fermentation in which respiration of cells was stimulated with hemin. Response surface model was able to explain the changes of the nisin production of L. lactis N8 in fed-batch fermentation system with high fidelity (R-2 98%) and insignificant lack of fit. Accordingly, the equation developed indicated the optimum parameters for glucose, hemin, and dissolved oxygen were 8 g L-1 h(-1), 3 mu g mL(-1) and 40%, respectively. While 1711 IU mL(-1) nisin was produced by L. lactis N8 in control fed-batch fermentation, 5410 IU mL(-1) nisin production was achieved within the relevant optimum parameters where the respiration of cell was stimulated with hemin. Accordingly, nisin production was enhanced 3.1 fold in fed-batch fermentation using hemin. In conclusion the nisin production of L. lactis N8 was enhanced extensively as a result of increasing the biomass by stimulating the cell respiration with adding the hemin in the fed-batch fermentation. (C) 2015 American Institute of Chemical Engineers