Disposable and ultrasensitive label-free gold nanoparticle patterned poly(3,4-ethylenedioxythiophene-co-3-methylthiophene) electrode for electrochemical immunosensing of prostate-specific antigen


Uruc S., Dokur E., GÖRDÜK Ö., ŞAHİN Y.

New Journal of Chemistry, vol.48, no.23, pp.10415-10426, 2024 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 48 Issue: 23
  • Publication Date: 2024
  • Doi Number: 10.1039/d4nj02034k
  • Journal Name: New Journal of Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Biotechnology Research Abstracts, Chemical Abstracts Core, Chimica, Compendex, DIALNET
  • Page Numbers: pp.10415-10426
  • Yıldız Technical University Affiliated: Yes

Abstract

This study introduces a novel label-free immunosensor that has the potential to be utilized for clinical screening and diagnosis of prostate cancer. The electrochemical immunosensor's performance was enhanced by combining 3,4-ethylenedioxythiophene (EDOT), 3-methylthiophene (3MT), and gold nanoparticles (AuNPs), enabling the accurate and sensitive determination of serum prostate specific antigen (PSA) concentrations. The immunosensor platform used [Fe(CN)6]3−/4− as a redox probe to obtain the signal, and the DPV technique was used to correlate the signal with the PSA concentration. The linear range of AuNPs/p(EDOT-co-3MT)/PGE is 0.1 to 50 000 pg mL−1, with a detection limit of 0.083 pg mL−1. In interference studies with interferences such as bovine serum albumin, carcinoembryonic antigen, uric acid, ascorbic acid, vitamin B12, vitamin B9, l-tyrozine, l-phenylalanine, Cu2+, Fe2+, and Zn2+ the immunosensor showed excellent anti-interference properties. The designed sensor platform is facile, inexpensive, highly sensitive, and selective, and has a high surface area, excellent electrical conductivity, and biocompatibility. Furthermore, this technology is highly suitable for industrial-scale production and can be easily converted into point-of-care detection devices due to its low cost, reproducibility, selectivity, and accuracy.