The purpose of this study was to synthesize strontium substituted melt derived 45S5 bioactive glasses by use of rice hull ash silica and commercial silica. The degradation behaviour of bioactive glass samples was studied through the weight loss in the tris-(hydroxymethyl)-aminomethane solution. The in vitro bioactivity of the bioactive glass samples was evaluated by soaking studies in simulated body fluid. The apatite like layer formation on glasses surface was assessed by X-ray powder diffraction, scanning electron microscope, inductively coupled plasma and Vickers hardness measurement analyses. Also, the effect of bioactive glass samples on osteoblasts in vitro and cell mineralisation was investigated. The present investigation revealed that both of the samples show similar bioactivity but 45S5 bioactive glass from rice hull ash silica supported cell activity better than commercial silica. Thus, rice hull ash silica can be used to synthesize strontium substituted 45S5 bioactive glasses and it is also good alternative to commercial silica.