Abilities of partial least-squares (PLS-2) multivariate calibration in the analysis of quaternary mixture of food Colors (E-110, E-122, E-124, E-131)

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Keser Karaoglan G., Guemruekcue G., Oezguer M. U., Bozdogan A. E., Asci B.

ANALYTICAL LETTERS, vol.40, no.10, pp.1893-1903, 2007 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 40 Issue: 10
  • Publication Date: 2007
  • Doi Number: 10.1080/00032710701384675
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1893-1903
  • Yıldız Technical University Affiliated: Yes


Sunset Yellow (SY), Carmoisine (C), Ponceau 4R (P), and Patent Blue V (PB) are synthetic organic dyes which are under governmental regulations all over the world because of their toxicity and carcinogenicity. In this study, a simple and fast analytical procedure was proposed for the simultaneous determination of food dyes (SY, C, P, and PB) in powder drinks by means the partial least-square treatment of spectrophotometric absorbance between 450 – 730 nm, taken at 10 nm intervals. The experimental calibration matrix was constructed with 27 samples. The concentration ranges considered were 2, 3, 4 mg . ml21 for SY, 7, 8, 9 mg . ml21 for C, 9, 10, 11 mg . ml21 for P, and 0.3, 0.4, 0.5 mg . ml21 for PB. The method was applied to the determination of dyes in different commercially available powder drinks. The results obtained by the application of the PLS-2 method were statistically compared with those obtained by an HPLC method using the F and t tests. Very similar values were found by two methods. No time consuming pretreatment was needed and this method also provides rapid, accurate and economical analysis of these colors.